Theme: Functions of biogenic amines. Ammonia formation in the organism and the path of its neutralization.

1. A place of carrying out of study, equipment:

- Department of Natural science, studying room;

- Evident posters and banners;

- Tables;

- Distributing stuffs;

- Panel of reagents;

- Panel of laboratory ware;

- Multimedia, overhead projector, PhEC, cuvette with 1 cm of thickness

2. Duration of studying of a theme:

     - 4 hours.

3. Study purpose:

Explain to students importance of processes of decarboxylation of amino acids, functions of biogenic amines and a path of their neutralisation. Histaminum role in development of allergic and inflammatory reactions. Paths of formation and neutralisation of ammonia, the development cause of hyperammonemia. Use of the received knowledge in diagnostics and treatment of various pathologies.

Tasks:

Student should know:

- Decarboxylation of amino acids

- Functions of biogenic amines and a path of their neutralisation

- Histaminum, its role in development of allergic and inflammatory processes

- Antihistamine drugs

- Paths of neutralisation of ammonia

- Synthesis of urea and its connection with transamination reactions

- Disturbance of synthesis and removing of urea from an organism

- Hyperammonemia, its causes and a consequence

Student should be able:

To perform laboratory work - urea definition in blood serum.

4. Motivation:

Studying of function of biogenic amines, ammonia formation in an organism and a path of its neutralisation, hence, to study biochemical bases of development of diseases and methods of their treatment. Disturbance of these processes lead to morbid conditions. This knowledge for the future doctors of the general of a profile for statement of the diagnosis and diseases.

5. Communications between subject and inside subject:

Knowledge of composition and properties of a connective tissue, and also biosynthesis and pathologies bound to their changes is based on knowledge on bioorganic chemistry, physiology, anatomy, histology. This knowledge is necessary for mastering further such subjects, as pathological physiology, pharmacology, therapy, surgery, rheumatology, etc. clinical disciplines.